Abstract
There is evidence that the dopaminergic system is sensitive to altered po2 in the immature brain. However, the respective enzyme activities have not been measured in the living neonatal brain together with brain oxidative metabolism. Therefore 18F-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with positron emission tomography (PET) was used to estimate the activity of the aromatic amino acid decarboxylase (AADC) in the brain of fifteen newborn piglets (2-5 days old). Two PET scans were performed in each piglet. Eleven animals underwent a period of normoxia and moderate hypoxia/hypercapnia (H/H). The remaining four animals were used as an untreated control group. Simultaneously, the brain tissue po2 was recorded, the regional cerebral blood flow (CBF) was measured with colored microspheres and the cerebral metabolic rate of oxygen (CMRO2) was determined. In addition, in four untreated and six H/H treated piglets the relative amounts of fluorodopamine and the respective metabolites were determined in brain tissue samples using HPLC analysis. H/H conditions were induced by lowering the inspired fraction of oxygen from 0.35 to 0.10 and adding CO2 to the inspired gas resulting in an arterial pco2 between 74 and 79 mmHg. H/H elicited a more than 3-fold increase of the CBF (P
Original language | English |
---|---|
Pages (from-to) | 23-33 |
Number of pages | 10 |
Journal | Brain research |
Volume | 934 |
Issue number | 1 |
DOIs | |
Publication status | Published - 26 Apr 2002 |
Keywords
- Aromatic amino acid decarboxylase
- Colored microsphere
- Dopamine metabolism
- FDOPA
- Newborn piglet
- Positron emission tomography
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Bauer, R., Brust, P., Walter, B., Vorwieger, G., Bergmann, R., Elhalag, E., Fritz, A., Steinbach, J., Füchtner, F., Hinz, R., Zwiener, U., & Johannsen, B. (2002). Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets. Brain research, 934(1), 23-33. https://doi.org/10.1016/S0006-8993(02)02315-6
Bauer, Reinhard ; Brust, Peter ; Walter, Bernd et al. / Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets. In: Brain research. 2002 ; Vol. 934, No. 1. pp. 23-33.
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title = "Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets",
abstract = "There is evidence that the dopaminergic system is sensitive to altered po2 in the immature brain. However, the respective enzyme activities have not been measured in the living neonatal brain together with brain oxidative metabolism. Therefore 18F-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with positron emission tomography (PET) was used to estimate the activity of the aromatic amino acid decarboxylase (AADC) in the brain of fifteen newborn piglets (2-5 days old). Two PET scans were performed in each piglet. Eleven animals underwent a period of normoxia and moderate hypoxia/hypercapnia (H/H). The remaining four animals were used as an untreated control group. Simultaneously, the brain tissue po2 was recorded, the regional cerebral blood flow (CBF) was measured with colored microspheres and the cerebral metabolic rate of oxygen (CMRO2) was determined. In addition, in four untreated and six H/H treated piglets the relative amounts of fluorodopamine and the respective metabolites were determined in brain tissue samples using HPLC analysis. H/H conditions were induced by lowering the inspired fraction of oxygen from 0.35 to 0.10 and adding CO2 to the inspired gas resulting in an arterial pco2 between 74 and 79 mmHg. H/H elicited a more than 3-fold increase of the CBF (P",
keywords = "Aromatic amino acid decarboxylase, Colored microsphere, Dopamine metabolism, FDOPA, Newborn piglet, Positron emission tomography",
author = "Reinhard Bauer and Peter Brust and Bernd Walter and Gerd Vorwieger and Ralf Bergmann and Elsayed Elhalag and Anne Fritz and J{\"o}rg Steinbach and Frank F{\"u}chtner and Rainer Hinz and Ulrich Zwiener and Bernd Johannsen",
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day = "26",
doi = "10.1016/S0006-8993(02)02315-6",
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volume = "934",
pages = "23--33",
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Bauer, R, Brust, P, Walter, B, Vorwieger, G, Bergmann, R, Elhalag, E, Fritz, A, Steinbach, J, Füchtner, F, Hinz, R, Zwiener, U & Johannsen, B 2002, 'Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets', Brain research, vol. 934, no. 1, pp. 23-33. https://doi.org/10.1016/S0006-8993(02)02315-6
Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets. / Bauer, Reinhard; Brust, Peter; Walter, Bernd et al.
In: Brain research, Vol. 934, No. 1, 26.04.2002, p. 23-33.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets
AU - Bauer, Reinhard
AU - Brust, Peter
AU - Walter, Bernd
AU - Vorwieger, Gerd
AU - Bergmann, Ralf
AU - Elhalag, Elsayed
AU - Fritz, Anne
AU - Steinbach, Jörg
AU - Füchtner, Frank
AU - Hinz, Rainer
AU - Zwiener, Ulrich
AU - Johannsen, Bernd
PY - 2002/4/26
Y1 - 2002/4/26
N2 - There is evidence that the dopaminergic system is sensitive to altered po2 in the immature brain. However, the respective enzyme activities have not been measured in the living neonatal brain together with brain oxidative metabolism. Therefore 18F-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with positron emission tomography (PET) was used to estimate the activity of the aromatic amino acid decarboxylase (AADC) in the brain of fifteen newborn piglets (2-5 days old). Two PET scans were performed in each piglet. Eleven animals underwent a period of normoxia and moderate hypoxia/hypercapnia (H/H). The remaining four animals were used as an untreated control group. Simultaneously, the brain tissue po2 was recorded, the regional cerebral blood flow (CBF) was measured with colored microspheres and the cerebral metabolic rate of oxygen (CMRO2) was determined. In addition, in four untreated and six H/H treated piglets the relative amounts of fluorodopamine and the respective metabolites were determined in brain tissue samples using HPLC analysis. H/H conditions were induced by lowering the inspired fraction of oxygen from 0.35 to 0.10 and adding CO2 to the inspired gas resulting in an arterial pco2 between 74 and 79 mmHg. H/H elicited a more than 3-fold increase of the CBF (P
AB - There is evidence that the dopaminergic system is sensitive to altered po2 in the immature brain. However, the respective enzyme activities have not been measured in the living neonatal brain together with brain oxidative metabolism. Therefore 18F-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with positron emission tomography (PET) was used to estimate the activity of the aromatic amino acid decarboxylase (AADC) in the brain of fifteen newborn piglets (2-5 days old). Two PET scans were performed in each piglet. Eleven animals underwent a period of normoxia and moderate hypoxia/hypercapnia (H/H). The remaining four animals were used as an untreated control group. Simultaneously, the brain tissue po2 was recorded, the regional cerebral blood flow (CBF) was measured with colored microspheres and the cerebral metabolic rate of oxygen (CMRO2) was determined. In addition, in four untreated and six H/H treated piglets the relative amounts of fluorodopamine and the respective metabolites were determined in brain tissue samples using HPLC analysis. H/H conditions were induced by lowering the inspired fraction of oxygen from 0.35 to 0.10 and adding CO2 to the inspired gas resulting in an arterial pco2 between 74 and 79 mmHg. H/H elicited a more than 3-fold increase of the CBF (P
KW - Aromatic amino acid decarboxylase
KW - Colored microsphere
KW - Dopamine metabolism
KW - FDOPA
KW - Newborn piglet
KW - Positron emission tomography
U2 - 10.1016/S0006-8993(02)02315-6
DO - 10.1016/S0006-8993(02)02315-6
M3 - Article
C2 - 11937066
SN - 0006-8993
VL - 934
SP - 23
EP - 33
JO - Brain research
JF - Brain research
IS - 1
ER -
Bauer R, Brust P, Walter B, Vorwieger G, Bergmann R, Elhalag E et al. Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets. Brain research. 2002 Apr 26;934(1):23-33. doi: 10.1016/S0006-8993(02)02315-6