Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets (2024)

Abstract

There is evidence that the dopaminergic system is sensitive to altered po2 in the immature brain. However, the respective enzyme activities have not been measured in the living neonatal brain together with brain oxidative metabolism. Therefore 18F-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with positron emission tomography (PET) was used to estimate the activity of the aromatic amino acid decarboxylase (AADC) in the brain of fifteen newborn piglets (2-5 days old). Two PET scans were performed in each piglet. Eleven animals underwent a period of normoxia and moderate hypoxia/hypercapnia (H/H). The remaining four animals were used as an untreated control group. Simultaneously, the brain tissue po2 was recorded, the regional cerebral blood flow (CBF) was measured with colored microspheres and the cerebral metabolic rate of oxygen (CMRO2) was determined. In addition, in four untreated and six H/H treated piglets the relative amounts of fluorodopamine and the respective metabolites were determined in brain tissue samples using HPLC analysis. H/H conditions were induced by lowering the inspired fraction of oxygen from 0.35 to 0.10 and adding CO2 to the inspired gas resulting in an arterial pco2 between 74 and 79 mmHg. H/H elicited a more than 3-fold increase of the CBF (P

Original languageEnglish
Pages (from-to)23-33
Number of pages10
JournalBrain research
Volume934
Issue number1
DOIs
Publication statusPublished - 26 Apr 2002

Keywords

  • Aromatic amino acid decarboxylase
  • Colored microsphere
  • Dopamine metabolism
  • FDOPA
  • Newborn piglet
  • Positron emission tomography

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  • Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets (1)

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Bauer, R., Brust, P., Walter, B., Vorwieger, G., Bergmann, R., Elhalag, E., Fritz, A., Steinbach, J., Füchtner, F., Hinz, R., Zwiener, U., & Johannsen, B. (2002). Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets. Brain research, 934(1), 23-33. https://doi.org/10.1016/S0006-8993(02)02315-6

Bauer, Reinhard ; Brust, Peter ; Walter, Bernd et al. / Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets. In: Brain research. 2002 ; Vol. 934, No. 1. pp. 23-33.

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title = "Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets",

abstract = "There is evidence that the dopaminergic system is sensitive to altered po2 in the immature brain. However, the respective enzyme activities have not been measured in the living neonatal brain together with brain oxidative metabolism. Therefore 18F-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with positron emission tomography (PET) was used to estimate the activity of the aromatic amino acid decarboxylase (AADC) in the brain of fifteen newborn piglets (2-5 days old). Two PET scans were performed in each piglet. Eleven animals underwent a period of normoxia and moderate hypoxia/hypercapnia (H/H). The remaining four animals were used as an untreated control group. Simultaneously, the brain tissue po2 was recorded, the regional cerebral blood flow (CBF) was measured with colored microspheres and the cerebral metabolic rate of oxygen (CMRO2) was determined. In addition, in four untreated and six H/H treated piglets the relative amounts of fluorodopamine and the respective metabolites were determined in brain tissue samples using HPLC analysis. H/H conditions were induced by lowering the inspired fraction of oxygen from 0.35 to 0.10 and adding CO2 to the inspired gas resulting in an arterial pco2 between 74 and 79 mmHg. H/H elicited a more than 3-fold increase of the CBF (P",

keywords = "Aromatic amino acid decarboxylase, Colored microsphere, Dopamine metabolism, FDOPA, Newborn piglet, Positron emission tomography",

author = "Reinhard Bauer and Peter Brust and Bernd Walter and Gerd Vorwieger and Ralf Bergmann and Elsayed Elhalag and Anne Fritz and J{\"o}rg Steinbach and Frank F{\"u}chtner and Rainer Hinz and Ulrich Zwiener and Bernd Johannsen",

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doi = "10.1016/S0006-8993(02)02315-6",

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Bauer, R, Brust, P, Walter, B, Vorwieger, G, Bergmann, R, Elhalag, E, Fritz, A, Steinbach, J, Füchtner, F, Hinz, R, Zwiener, U & Johannsen, B 2002, 'Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets', Brain research, vol. 934, no. 1, pp. 23-33. https://doi.org/10.1016/S0006-8993(02)02315-6

Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets. / Bauer, Reinhard; Brust, Peter; Walter, Bernd et al.
In: Brain research, Vol. 934, No. 1, 26.04.2002, p. 23-33.

Research output: Contribution to journalArticlepeer-review

TY - JOUR

T1 - Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets

AU - Bauer, Reinhard

AU - Brust, Peter

AU - Walter, Bernd

AU - Vorwieger, Gerd

AU - Bergmann, Ralf

AU - Elhalag, Elsayed

AU - Fritz, Anne

AU - Steinbach, Jörg

AU - Füchtner, Frank

AU - Hinz, Rainer

AU - Zwiener, Ulrich

AU - Johannsen, Bernd

PY - 2002/4/26

Y1 - 2002/4/26

N2 - There is evidence that the dopaminergic system is sensitive to altered po2 in the immature brain. However, the respective enzyme activities have not been measured in the living neonatal brain together with brain oxidative metabolism. Therefore 18F-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with positron emission tomography (PET) was used to estimate the activity of the aromatic amino acid decarboxylase (AADC) in the brain of fifteen newborn piglets (2-5 days old). Two PET scans were performed in each piglet. Eleven animals underwent a period of normoxia and moderate hypoxia/hypercapnia (H/H). The remaining four animals were used as an untreated control group. Simultaneously, the brain tissue po2 was recorded, the regional cerebral blood flow (CBF) was measured with colored microspheres and the cerebral metabolic rate of oxygen (CMRO2) was determined. In addition, in four untreated and six H/H treated piglets the relative amounts of fluorodopamine and the respective metabolites were determined in brain tissue samples using HPLC analysis. H/H conditions were induced by lowering the inspired fraction of oxygen from 0.35 to 0.10 and adding CO2 to the inspired gas resulting in an arterial pco2 between 74 and 79 mmHg. H/H elicited a more than 3-fold increase of the CBF (P

AB - There is evidence that the dopaminergic system is sensitive to altered po2 in the immature brain. However, the respective enzyme activities have not been measured in the living neonatal brain together with brain oxidative metabolism. Therefore 18F-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with positron emission tomography (PET) was used to estimate the activity of the aromatic amino acid decarboxylase (AADC) in the brain of fifteen newborn piglets (2-5 days old). Two PET scans were performed in each piglet. Eleven animals underwent a period of normoxia and moderate hypoxia/hypercapnia (H/H). The remaining four animals were used as an untreated control group. Simultaneously, the brain tissue po2 was recorded, the regional cerebral blood flow (CBF) was measured with colored microspheres and the cerebral metabolic rate of oxygen (CMRO2) was determined. In addition, in four untreated and six H/H treated piglets the relative amounts of fluorodopamine and the respective metabolites were determined in brain tissue samples using HPLC analysis. H/H conditions were induced by lowering the inspired fraction of oxygen from 0.35 to 0.10 and adding CO2 to the inspired gas resulting in an arterial pco2 between 74 and 79 mmHg. H/H elicited a more than 3-fold increase of the CBF (P

KW - Aromatic amino acid decarboxylase

KW - Colored microsphere

KW - Dopamine metabolism

KW - FDOPA

KW - Newborn piglet

KW - Positron emission tomography

U2 - 10.1016/S0006-8993(02)02315-6

DO - 10.1016/S0006-8993(02)02315-6

M3 - Article

C2 - 11937066

SN - 0006-8993

VL - 934

SP - 23

EP - 33

JO - Brain research

JF - Brain research

IS - 1

ER -

Bauer R, Brust P, Walter B, Vorwieger G, Bergmann R, Elhalag E et al. Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets. Brain research. 2002 Apr 26;934(1):23-33. doi: 10.1016/S0006-8993(02)02315-6

Effect of hypoxia/hypercapnia on metabolism of 6-[18F]fluoro-L-DOPA in newborn piglets (2024)
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